eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
Current issue Archive Manuscripts accepted About the journal Special Issues Editorial board Abstracting and indexing Subscription Contact Instructions for authors Publication charge Ethical standards and procedures
Editorial System
Submit your Manuscript
SCImago Journal & Country Rank
1-2/2006
vol. 31
 
Share:
Share:
abstract:

Clinical immunology
Determination of monocyte count by hematological analyzers, manual method and flow cytometry in polish population

Elżbieta Górska
,
Urszula Demkow
,
Roman Pińkowski
,
Barbara Jakubczak
,
Dorota Matuszewicz
,
Jolanta Gawęda
,
Wioletta Rzeszotarska
,
Maria Wąsik

(Centr Eur J Immunol 2006; 31 (1-2): 1-5)
Online publish date: 2006/10/12
View full text Get citation
 
The aim of the study was to compare the absolute and relative monocyte count obtained by microscopic method (M), flow cytometry (FC) and the automated hematological analyzers (HA) as well as to validate of reference ranges of monocytes count in polish population. Blood samples from 84 healthy adults were analyzed. The cell counts determined by three HA: two HMX and one Cell-Dyn were confronted with M and FC. The counts exceeding 800/ul were found in 4.8%, 5.9%, 15.5% and 13.1% patient’s blood samples assessed by: M, FC, HMx and Cell-Dyn, respectively. The difference between both HA and M was significant (p<0.05). FC detection of relative count provided the closest results to M but difference between the means was significant. The staining anti CD45 and anti CD14 monoclonal antibodies gives the best agreement with the both HA than with M. All examined methods revealed significant percentage of monocytes above the upper limit of the reference range accepted as 8%. Our preliminary data indicate that the upper limit of the reference range of monocytes should be higher. There is a great need for cross-sectional population study to establish new reference range for monocytes in order to appropriately manage individuals with infection diseases and hematological disturbances.
keywords:

monocytes, counting, different methods

Quick links
© 2024 Termedia Sp. z o.o.
Developed by Bentus.