eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
Current issue Archive Manuscripts accepted About the journal Special Issues Editorial board Abstracting and indexing Subscription Contact Instructions for authors Publication charge Ethical standards and procedures
Editorial System
Submit your Manuscript
SCImago Journal & Country Rank
1-2/2006
vol. 31
 
Share:
Share:
abstract:

Experimental immunology
Immune mechanism of the retarded growth of tumor nodules in mice exposed to single low-level irradiations with X-rays

Aneta Cheda
,
Jolanta Wrembel-Wargocka
,
Ewa M. Nowosielska
,
Marek K. Janiak

(Centr Eur J Immunol 2006; 31 (1-2): 44-50)
Online publish date: 2006/10/12
View full text Get citation
 
A number of epidemiological and experimental data indicate that exposures to low doses of low-LET ionizing radiation may trigger the activity of natural anti-tumour immune mechanisms and inhibit tumour growth. Natural killer (NK) cells and activated macrophages play an important role in the anti-tumour defence of the host. In our experiments, BALB/c mice were irradiated with single doses of 0.1, 0.2, or 1.0 Gy X-rays and then intravenously (i.v.) injected with L1 sarcoma cells. Cytotoxic activities of NK cells and macrophages were estimated in vitro using the classical 51Cr-release and [3H] thymidine-uptake assays, respectively. The anti-asialo GM1 (GM1Ab) antibody and carrageenan (CGN) were intraperitoneally (i.p.) injected to block the NK cell- and macrophage-mediated activities in vivo, respectively. Whole body irradiation of mice with a single low dose (0.1 or 0.2 Gy) of X-rays led to a significant reduction of the number of tumour colonies induced in the lungs accompanied by the enhanced cytotoxic activities of both NK lymphocytes and macrophages. Treatment of mice with GM1Ab or CGN abrogated the tumour-inhibitory effect of the exposures to 0.1 and 0.2 Gy X-rays. The obtained data suggest that suppression of the development of pulmonary tumour colonies by single irradiations of mice with the two low doses of X-rays may result from stimulation of the natural anti-tumour defence reactions mediated by NK cells and/or cytotoxic macrophages.
keywords:

X rays, low doses, tumour colonies, macrophages, NK cells, cytotoxic activity

Quick links
© 2024 Termedia Sp. z o.o.
Developed by Bentus.