eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
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1/2016
vol. 41
 
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abstract:
Clinical immunology

Single antigen flow beads for identification of human leukocyte antigen antibody specificities in hypersensitized patients with chronic renal failure

Mustafa Soyöz
,
Tülay Kılıçaslan-Ayna
,
Aslı Özkızılcık-Koçyigit
,
Derya Güleç
,
Ibrahim Pirim

(Cent Eur J Immunol 2016; 41 (1): 93-100)
Online publish date: 2016/01/20
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Aims of this study were to identify class I and class II antibodies in highly sensitized patients by flow cytometry single antigen bead (FC-SAB) assay and to evaluate according to donor HLA type in order to increase their kidney transplantation chance.

Material and methods: We analyzed 60 hypersensitive patients of 351 individuals, who applied to our laboratory for PRA test in November 2013-December 2014. Flow cytometric PRA screening and single antigen bead commercial kits were used for these analyses.

Results: In our study group, 19 (31.7%) of these patients were male while 41 (68.3%) patients were female. The most common acceptable antigens were A*02 (10.11%), HLA-A*23 (10.11%), HLA-B*38 (8.79%) and HLA-DRB1*03 (7.83%) in hypersensitive patients. The highest antibody reactivity on SAB was observed against HLA-A*25, HLA-B*45, HLA-DRB1*04 and HLA-DRB1*08 antigens.

Conclusions: The determination of these acceptable and unacceptable antigens may increase their transplantation chance. Pre-transplant HLA antibody identifications provide prognostic information with respect to the determination of patients who are at increased risk of graft loss.

keywords:

flow cytometry, DSA, single antigen bead, HLA

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